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Avian Pathogen

Avian Pathogen Sampling for PCR testing

Objective

  • To detect opportunistic human pathogens transmitted by birds and bats (Cryptococcus neoformans, Histoplasma capsulatum, and Chlamydophila psittaci) in soil, swab samples, or animal droppings.

Please note: Take caution while handling samples containing human pathogens!
The Identification of H. capsulatum by PCR is qualitative (reported as "Detected" or "Not detected"). C. neoformans and C. psittaci are quantitative.

Advantages and Disadvantages

  • Accurate detection and identification
  • Quick turnaround time

Equipment

  • Clean or sterile containers or Ziploc bags for bulk samples.
  • Clean or sterile containers for water samples.
  • DustChek™ or Mitest™ sampler (attachment for standard household vacuum cleaners) for dust sampling.
  • 3-pc air sampling cassette with polycarbonate filter and vacuum pump for air sampling.

Sampling

  • Swab samples can be collected with liquid swabs from suspected area.
  • For air samples from indoor areas follow same sampling procedures as for other PCR analyses.
  • For bulk samples collect bird droppings or dust or soil from suspected area. 1 gram of material is sufficient for the testing.
  • Collect water from suspected areas.

Shipping

  • Air cassettes, bulks and swab samples should be shipped to the laboratory for overnight delivery.