To detect viable and non-viable cells of Legionella pneumophila and/or Legionella species* in potable and non-potable water or from swab samples. The PCR test can be a valuable tool to quickly identify a source of Legionella bacteria in an outbreak situation. We recommend following up with culture analysis because PCR can not differentiate between viable and non-viable cells.
Advantages and Disadvantages
Fast and accurate detection of Legionella bacteria.
PCR test can not differentiate between viable and non-viable cells.
Clean collection bottles (if culture testing is requested. In addition sodium thiosulfate should be added to neutralize effects of chlorine)
Collect a minimum of 100 ml water for PCR testing. If culture testing and PCR are requested, follow sampling instructions for culture test.
Swab inside of shower heads, faucets, and other surfaces that are likely to contain bacteria using the pre-wetted swab pad. Take second swab for culture test from the same area if follow up with culture is requested.
Ship samples for overnight delivery on ice packs to the laboratory.