1. Sampling Overview
    1. Culturable air samples
    2. Non-culturable air samples
    3. Surface samples
    4. Special cases
  2. Interpretation Overview
    1. Activity levels
    2. Weather conditions
    3. Condition of the area sampled
  3. Additional Information
    1. Spore trap air sampling
    2. Andersen or Biocassette air sampling
    3. Surface sampling (Tape, Swab, Bulk)
    4. Surface sampling (Dust)
  4. Environmental Reporter
  5. Ask Dr. Burge
  6. Allergen Glossary
  7. Food Microbiology Glossary
  8. Fungal Glossary
  9. Resources
 

Surface Sampling (Dust)

Objective

To determine whether the surface sampled indicates a skewing in the normal distribution of spore types, and also note for "marker" genera, which may indicate indoor mold growth.

Note: A dust sample can be analyzed by direct microscopic examination or by culturing. Both have their own advantages and disadvantages. It is usually recommended that both culture and direct examination be done on the sample as either of the analysis done alone may result in an inaccurate characterization of the dust sampled. If only one method of analysis is to be used, usually a culturable analysis is the best choice.

Advantages and Disadvantages

Advantages

Dust samples are felt to relate to the history of what is in the air.

Dust sample for a direct examination may be analyzed immediately.

Disadvantages

The results are dependent upon the sampling method and results may be difficult to interpret.

Equipment

Dust filter cassette.

Vacuum pump.

Sampling protocols

Control as many sampling variables as possible including the time of vacuuming, the amount of pressure applied, the size of the area sampled, the direction of sampling, and the equipment used.

Sample from areas that are unlikely to be affected by foot traffic or other sources of fungi that are not from the air. Corners of rooms are often good areas.

Try to get at least a teaspoon of dust and record the area vacuumed.

Shipping

There are no special shipping requirements for fungal analysis (dust samples for allergen analysis should be sent with a cold back via an overnight courier).